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Protein-siRNA Conjugate Development

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CD Formulation's experienced team specializes in bioconjugation technology, offering services that link oligonucleotides with various proteins, such as fluorescent, carrier proteins, and enzymes. Their technical process encompasses modification, activation, and conjugation to ensure high-quality conjugate products. Additionally, they tailor services based on customer requirements, demonstrating their technical expertise and flexibility.

Why Conjugate Proteins with siRNA?

Protein-siRNA conjugates serve a vital role in biotech and molecular research. siRNA, a key molecular tool for gene silencing and expression regulation, can be conjugated with specific proteins to boost cellular stability, targeting precision, and delivery efficiency. This method is commonly used to study cellular signal transduction, gene function analysis, and the exploration of protein interaction mechanisms. With the integration of maleimide/thiol cross-linking techniques, researchers can achieve efficient and specific conjugation, driving innovation in molecular biology.

Explore Our Services for Protein-siRNA Conjugate Development

CD Formulation specializes in developing protein-siRNA conjugates, focusing on optimizing delivery systems. Their expert team employs advanced conjugation techniques to bind oligonucleotides with various proteins, enhancing delivery precision and efficiency. Additionally, they emphasize adapting to specific client needs, offering tailored solutions that demonstrate their leading position in drug delivery technology.

Categories of Proteins Suitable for Conjugation

Items	Descriptions
Catalytic Proteins	When linked with siRNA, these proteins can bolster the control of particular gene activities, assisting scientists in enhancing reaction processes.
Cellular Framework Proteins	Engaged in constructing cellular frameworks and preserving structural stability, these proteins, when conjugated with siRNA, facilitate the exploration of how cell shape correlates with function.
Carrier Proteins	On pairing with siRNA, carrier proteins provide a deeper insight into their functions in molecular transport and signaling pathways, thus allowing for more focused molecular interventions.
Signal Transduction Proteins	These siRNA conjugates assist in dissecting the mechanisms of signal pathways and their management networks, thereby advancing research in molecular biology.
Recognition Proteins	When combined with siRNA, recognition proteins present novel angles for examining molecular interaction and binding precision, which contribute to a better understanding of intricate biological identification mechanisms.

Our Workflow of Protein-siRNA Conjugate Development

Fig.1 Development flowchart of protein-siRNA conjugates. Fig.1 Flow chart of protein-siRNA conjugate development. (CD Formulation)

Protein and siRNA Preparation

Modify and activate the selected protein and siRNA to facilitate effective conjugation.

Conjugation Process

Employ bio-conjugation techniques to link the siRNA with the protein, ensuring optimal interaction and stability.

Quality Control

Conduct thorough testing to verify the integrity, efficacy, and stability of the conjugated product.

Customization and Optimization

Fine-tune the conjugates based on client needs, optimizing delivery efficiency and targeting accuracy for specific applications.

Our Technology Platforms

Solid-Phase Synthesis	Enzymatic Synthesis
Solid-phase synthesis uses a chemical approach to methodically assemble oligonucleotides on a solid substrate. This enables precise control over nucleotide sequence and chemical alterations, ensuring the resulting siRNA has significant stability.	Enzymatic synthesis depends on biological enzymes to facilitate the extension of nucleic acid chains. By leveraging the efficient catalytic properties of natural enzymes, desired siRNA molecules can be swiftly produced in lab settings.

Advantages of Protein-siRNA Conjugate Development Services

The conjugation process enables precise delivery and targeting of siRNA within biological systems.
Services offer flexibility to tailor conjugates according to specific client needs and experimental requirements.
Advanced techniques ensure high-quality conjugate products that meet rigorous standards.
Leveraging a skilled team specializing in bio-conjugation technologies guarantees effective and innovative solutions.
Enhances the delivery system efficiency by combining siRNA with specific proteins, thereby improving effectiveness in applications.

Published Data

Technology: Development of Oligonucleotide-Protein Conjugates Using a Drug Delivery Platform

Journal: ChemistryOpen

IF: 2.5

Published: 2019

Results:

Researchers have developed a cutting-edge method for connecting bifunctional linkers to thiol-oligonucleotides, forming active intermediates for protein attachment. This new method offers similar bonding efficiency to traditional techniques, which attach linkers directly to proteins, but with improved linker activity and batch consistency. Another technique involves directly bonding oligonucleotides with carboxyl groups. Both methods are useful for modifying proteins, amino-containing polymers, and aminolipids, facilitating the development of complex therapeutic nucleic acid delivery systems.

Fig.2 Synthesis of protein conjugates with oligonucleotide-FdU. Fig.2 Preparation of oligonucleotide-FdU protein conjugates. (Aviñó A, et al., 2019)

CD Formulation is dedicated to providing high-quality support for your research and innovation needs. For more information or personalized consultation, please feel free to contact us.

References

Aviñó A, Unzueta U, Virtudes Cespedes M, et al. Efficient bioactive oligonucleotide-protein conjugation for cell-targeted cancer therapy. ChemistryOpen. 2019, 8(3): 382-387.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.

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