CD Formulation offers our customers the opportunity to modify the phosphate backbone in customized nucleic acid molecules. This modification is noteworthy as it involves the establishment of a specific bond between bases, rather than the incorporation of a particular chemical entity, such as an unconventional base or quencher.

Why Perform Phosphate Backbone Modifications on Nucleic Acids?

Nucleic acid optimization necessitates modification, with backbone modification being one of the more prevalent approaches. Among these methods, phosphorothioate modifications serve as a representative example of nucleic acid optimization. In this method, sulfur atoms substitute the non-bridging oxygen atoms in the phosphodiester bonds of nucleotides, resulting in P-S bonds rather than P-O bonds.

Advantages of modification for nucleic acids

• The hydrophilicity of the oligonucleotide is reduced
• Provides resistance to nuclease degradation
• Enhances the stability of drugs
• Enhancement of drug half-life

Fig.1 Phosphate-backbone modifications of siRNA. (Ali Zaidi S S, et al., 2023)

Explore Our Phosphate Backbone Modification Services

CD Formulation provides services to modify nucleic acid phosphate backbones, enhancing their stability and functionality.

General Workflow for Phosphate Backbone Modification

Fig.2 Flow chart of phosphate backbone modification. (CD Formulation)

• Nucleic Acid Synthesis

Customers can provide us with their requirements and we can customize nucleic acid drug products for them.

• Selection of Modification Type

We have a wide range of retouching tools to choose from, depending on the target requirements.

• Modification Reaction

Incorporate the requisite modification reagents to facilitate the chemical reaction under designated reaction conditions.

• Purification

Modified nucleic acids must be isolated and purified through methods like HPLC.

• Characterization

Confirming the modification's success is crucial, employing techniques such as mass spectrometry, NMR, or HPLC.

Our Technology Platforms

Solid Phase Synthesis Technology Platform

The creation of altered nucleic acids is commonly accomplished using solid-phase synthesis, a standard approach in oligonucleotide production.

Enzymatic Synthesis Technology Platform

Modifying the phosphate backbone requires enzyme synthesis technology, allowing precise structuring of DNA or RNA through optimized nucleic acids.

Why Choose Us for Phosphate Backbone Modification Services?

• Optimize production to offer competitive pricing, helping customers balance cost and quality.
• Implement a quality management system to meet international standards and ensure product reliability.
• Use diverse phosphate modification technologies, like phosphorothioate and 2'-O-methylation, for varied research needs.
• Apply advanced purification techniques to ensure high purity and stability, improving experimental reliability.

Published Data

CD Formulation provides nucleic acid phosphate backbone modification services focusing on enhancing the stability and bioactivity of nucleic acids through various chemical modifications. Our services cover the entire process from design and synthesis to modification, purification, and characterization, ensuring that clients receive high-quality modified nucleic acids to meet research and drug development needs. Contact us, and we will offer customized solutions to assist in nucleic acid formulation development.