In Vitro ADME Assays for Nucleic Acid Drugs

Based on our state-of-the-art research, CD Formulation seeks to develop drugs through ADME studies. We are able to offer a wide range of in vitro ADME services and provide unrivaled experience and expertise in each area. Our company is a trusted partner and offers innovative research techniques.

Why In Vitro ADME Assays of Nucleic Acid Drugs?

ADME is the abbreviation for Absorption, Distribution, Metabolism and Excretion. The ADME properties of a drug are determined by its structure, and the determination of ADME properties of a drug can provide an important reference value for the prediction of its bioavailability and bioactivity (i.e., whether a drug can reach its target and produce corresponding therapeutic effects). As the potency of compounds increases during lead compounding and lead compound optimization, in vitro ADME assays provide the necessary data to gain insight into key physicochemical properties and structural motifs to ensure compound efficacy and safety.

Fig.1 Introduction to in vitro ADME assays. (CD Formulation)

Explore Our In Vitro ADME Analysis Services for Nucleic Acid Drugs

In recent years, our in vitro ADME research team has made progress on multiple fronts. A variety of advanced ultra-high throughput LC-MS/MS analytical techniques have been utilized in in vitro ADME studies, further improving the speed, quality and throughput of the tests.

Measuring the in vitro ADME properties of nucleic acid drugs can provide significant value in predicting their bioavailability and biological activity. With the support of our comprehensive in vitro ADME services platform, you can focus on optimizing your compounds and move to the next stage efficiently and confidently.

Our in vitro ADME Capabilities

Physical Chemical Properties

• Solubility, lipophilicity (LogD & LogP), pKa, buffer stability

Distribution

• Blood-to-plasma ratio
• Protein binding: equilibrium dialysis, ultracentrifugation, and ultrafiltration
• Tissue binding

Permeability

• PAMPA
• Caco-2, MDCK II, MDR1-MDCK II, BCRP-MDCK II, MDCK I, MDR1-MDCK I Monolayers

In Vitro Metabolism Studies

• Metabolite profiling and identification
• Metabolic stability assay
• Extrahepatic metabolism
• Reactive metabolite analysis
• Drug-drug interactions

CYP & Non-CYP Enzymes

• Cytochrome P450 (CYP) Reversible inhibition and time-dependent inhibition
• PXR/AhR Activation & CYP induction (activity and mRNA)
• CYP phenotyping
• UGT, FMO, SULT

Our In Vitro ADME Analysis Solutions for Nucleic Acid Drugs

Our in vitro services provide the data-driven support you need to design, select, and advance your compounds from discovery to achieving IND milestones. Whether you need early development testing (metabolic stability, kinetic and thermodynamic solubility, plasma protein binding, etc.) or a fully integrated program, our experts will work with your team to understand your program's needs and help you move toward success.

Experimental Validation of In Vitro ADME Analysis

In order to ensure the accuracy and reliability of our customers' experimental data, the standard experimental system for in vitro ADME has been validated:

• Standard experimental designs are validated using commercial compounds, which are highly consistent with data reported in the literature.
• Highly reproducible experimental data, control compounds have gone through thousands of experiments, and existing experimental systems are regularly validated with commercial compounds every year.
• When establishing new experimental platforms, we will go through a multi-dimensional examination to ensure the reliability of the experimental data, such as the degree of match with the reported data, day-to-day differences, batch differences, etc.

General Workflow for In Vitro ADME Analysis

Fig.2 Flow chart of in vitro ADME analysis. (CD Formulation)

• Sample Preparation - Nucleic acid drugs and biological samples for experiments were collected and processed for analysis using HPLC-MS.
• Solubility and Stability Test - Determine the solubility of nucleic acids at various pH levels and assess their chemical stability in biologically relevant environments.
• Metabolic Stability Test - Evaluate the metabolic stability of nucleic acid drugs by utilizing liver microsomes or hepatocyte culture systems to gain insights into their in vivo metabolism.
• Membrane Permeability Studies - Determine the absorption of nucleic acid drugs in the gastrointestinal tract to predict their bioavailability using Caco-2 cell models or artificial membrane systems.
• Data Analysis - A comprehensive statistical analysis and thorough interpretation of all experimental data are conducted to ensure the accuracy and reliability of the results.

Why Choose Us for In Vitro ADME Analysis?

• High-throughput and Comprehensive Types of Services
  We can provide all kinds of services from drug development to large-scale production, with a variety of experimental types, which is convenient for customers to make one-stop choices. Multiple automated instrumentation platforms can realize the continuous improvement of throughput.
• Rapid Data Delivery
  Relying on our strong technical capabilities, excellent operation services and constantly improving experimental throughput, we can save time and cost for our clients. For most screening phase experiments, it takes less than 5 working days from the receipt of compounds to the submission of project reports.
• High Quality Experimental Data
  We can provide a complete set of in vitro ADME evaluation services to meet the requirements of new drug clinical trial submission.
• Experienced Researchers
  Most of our staff members have postgraduate degrees, and our expert team has many years of experience in ADME research, which can provide effective data in a short period of time, saving resources for drug development projects and accelerating the development process.

Publication Data

CD Formulation has separate teams focusing on in vitro ADME experiments and analysis, with first-class experimental and analytical capabilities and project throughput to ensure rapid and high-quality delivery, helping you to complete ADME and pharmacokinetic testing services. If you have any questions, please contact our staff and we will be at your service as soon as possible.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.