Custom Single-Stranded DNA (ssDNA) Synthesis

Utilizing CD Formulation's state-of-the-art equipment, advanced technology, and experienced staff, we can help you complete your ssDNA customization project efficiently and economically without compromising quality.

What is ssDNA?

Single-stranded DNA (ssDNA) has proven to be an ideal template for knock-in experiments using CRISPR technology and it demonstrates superior editing efficiencies, reduced cytotoxicity, and fewer off-target effects. It has been validated across multiple insertion sites, accommodating a wide range of insertion sequences, and has been employed in various knock-in experiments involving human primary cells. Additionally, ssDNA has found extensive applications in single-stranded conformation polymorphism, in vitro transcription studies, S1 nuclease localization, probe preparation and labeling, differential subtractive hybridization, and other experimental contexts. For instance, ssDNA can serve as a scaffold in DNA nanotechnology, a carrier for drug delivery, a tool for molecular diagnostics, a medium for DNA-based data storage, and for various nanoscale applications.

Fig.1 Advantages of Single-Stranded DNA (ssDNA). (CD Formulation)

Explore Our Custom ssDNA Synthesis Services

CD Formulation provides custom synthesis services for ssDNA with a range of modifications, sizes, and purities. All nucleic acid molecules are synthesized using our advanced nucleic acid preparation technology platform and purified through HPLC. To guarantee the highest quality of ssDNA, each nucleic acid molecule is characterized using MS. Additionally, all ssDNA produced by our facility undergoes thorough analysis by HPLC and MS for quality control purposes.

Our Capabilities of Single-Stranded DNA (ssDNA) Synthesis

General Workflow for ssDNA Preparation

Fig.2 Flow chart of ssDNA preparation. (CD Formulation)

• DNA Extraction

The desired genes are extracted by using extraction reagents and the product is later purified.

• PCR Amplification

The target DNA fragments need to be amplified by specialized PCR to obtain the target product.

• Removal of Double Strands

The removal of double-stranded DNA can be accomplished using various methods. One commonly employed technique is base denaturation, while another method involves the isolation of ssDNA through denaturing polyacrylamide gel electrophoresis (PAGE).

• Purification of ssDNA

The ssDNA obtained is purified as needed. Common methods for purification include dialysis, ethanol precipitation, or the use of specialized kits.

• Validation of ssDNA

Quantify the obtained ssDNA using UV spectrometry or fluorescence quantification, and verify its purity and size through electrophoresis or other analytical methods.

Our Single-stranded DNA (ssDNA) Synthesis Technology Platforms

Our Advantages of Custom ssDNA Synthesis Services

• Flexible synthesis scales: 0.05 µmol, 0.1 µmol, 0.2 µmol, 0.4 µmol. Batch orders are available upon request.
• Multiple modifications are possible, including 5' modifications, 3' modifications, internal modifications, and various external modifications.
• Quality control: MALDI-TOF Mass Spectrometry for Quality Assurance
• Our flexible synthesis platform utilizes solid-phase and ligation technologies to synthesize highly complex single-stranded DNAs (ssDNAs).

Publication Data

CD Formulation is dedicated to supporting your nucleic acid drug molecule research with our expertise in high-quality modified ssDNA synthesis and exceptional technical support. If you are interested in our services, please do not hesitate to contact us.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.