Custom MicroRNA (miRNA) Synthesis

CD Formulation can utilize our professional researchers, advanced technology platforms, and rich experience to provide our clients with a wide range of nucleic acid drug discovery and development.

Why Custom Synthetic miRNA?

MicroRNA (miRNA) is a class of small non-coding RNA molecules, approximately 20-24 nucleotides in length, that regulates gene expression primarily by binding to specific messenger RNA (mRNA). miRNA can direct the RNA-induced silencing complex (RISC) to down-regulate gene expression at the post-transcriptional level through mechanisms such as mRNA degradation or translational repression. The specific type of silencing employed is determined by the characteristics of the target mRNA.

Fig.1 Biogenesis and mechanism of miRNAs. (Melone M A B, et al., 2018)

Explore Our Custom microRNAs (miRNAs) Synthesis Services

Custom miRNA Synthesis Services

Mature miRNAs are produced from longer primary transcripts that are cleaved and processed by a series of nuclease enzymes. miRNA synthesis can be accomplished through synthetic methods, such as optimized solid-phase synthesis and HPLC purification. Following standardized quality control (QC) testing, miRNAs of stable quality are synthesized.

Custom miRNA Mimics Synthesis Services

miRNA mimic products can be introduced into cells that naturally express these microRNAs to simulate their function. They are also used to confirm the connections between target genes and miRNAs.

Custom miRNA Inhibitors Synthesis Services

The chemically synthesized miRNA inhibitor can be transfected into cells by simply encapsulating it with transfection reagents, eliminating the need to construct vectors or concern about viral protection. The transfection efficiency can be monitored using a transfection control.

Custom miRNA Agomir Synthesis Services

miRNA Agomir is a specially labeled and chemically modified small double-stranded RNA that regulates the biological functions of target genes by mimicking endogenous miRNAs within the miRiSC complex. Our primary focus is on modifying the antisense strand of the miRNA Agomir, which includes a cholesterol modification at the 3' end, four thioskeleton modifications at the 3' end, two thioskeleton modifications at the 5' end, and complete methoxylation of the strand.

miRNA Antagomirs Synthesis Services

miRNA antagonists are small single-stranded Rnas designed based on mature miRNA sequences. They are prepared using a chemical synthesis method and is specifically labeled and chemically modified. This highly efficient blocker is specifically engineered to inhibit endogenous microRNA.

General Workflow for miRNA Preparation

Fig.2 Flow chart of miRNA preparation. (CD Formulation)

• Template Design

The first step in synthesizing miRNAs involves designing an appropriate DNA template. This template design necessitates careful consideration of the sequence, structure, and function of the miRNA.

• Precursor Synthesis

The designed DNA template is transcribed into an initial microRNA precursor (pri-miRNA) using T7 RNA polymerase or a similar enzyme.

• MicroRNA Generation

In vitro, the synthesized pri-miRNA is processed by nucleases. This process first removes excess sequences to generate pre-miRNA, which is then further processed to produce mature miRNA.

• Purification and Analysis

Synthesized miRNAs require purification to remove impurities, followed by analysis through sequencing or mass spectrometry to ensure they meet quality and purity standards.

• Storage

Purified miRNA is typically stored in a specific buffer to maintain its stability. Storage conditions generally require low temperatures (e.g., -80°C) to prolong its shelf life.

Our sgRNA Synthesis Technology Platforms

Why Choose Us for Customized miRNA Synthesis?

• Synthetic miRNA technology delivers accurate and consistent sequences, crucial for reproducibility and reliability in research and applications.
• Our scientists can synthesize specific miRNA sequences to target genes for regulation in various experimental protocols.
• Synthesis technology enables large-scale production, which is advantageous for projects that necessitate multiple miRNAs for high-throughput screening and experimentation.
• With professional synthesis services, researchers can save time and reduce the complexity associated with laboratory self-synthesis, allowing them to concentrate on subsequent experiments and data analysis.

Publication Data

CD Formulation offers services for the synthesis, modification, purification, and related bioinformatics analysis of microRNA to support research and drug development. Contact us, and we will assist customers in efficiently obtaining high-quality miRNA products for gene regulation and related biological research.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.