Custom DNAzyme Synthesis

DNAzyme is a single-stranded DNA fragment characterized by its high catalytic activity and structural recognition capabilities, which are achieved through in vitro molecular evolutionary techniques. The active site of DNAzyme can differentiate between substrates at the atomic level and catalyze similar reactions. Leveraging our advanced synthesis and analytical technology platform, CD Formulation offers tailored, comprehensive solutions for DNAzyme to expedite our customers' nucleic acid drug discovery and development processes.

Background of DNAzymes

DNAzymes, or catalytic DNA, are short, single-stranded DNA enzymes that fold into active three-dimensional structures, enabling various biochemical reactions. Compared to enzymes composed of proteins or RNA, DNAzymes are more stable and easier to synthesize and amplify in vitro due to their DNA chemical structure. Furthermore, DNAzymes can exhibit the same or even superior catalytic activity and specificity compared to other natural enzymes. As a result, DNAzymes have significant applications in biochemical fields such as bioimaging, detection, and sensing.

Explore Our Custom DNAzyme Synthesis Services

DNAzymes are derived from random sequence DNA using in vitro selection, a powerful method for isolating rare DNA or RNA sequences with specific functions from a large pool of single-stranded molecules.

DNAzyme Preparation through Binding Arm Design

The first method involves the rational design of the DNAzyme binding arm. However, the catalytic core of the DNAzyme must be known in advance. The sequences of the binding arms can be designed similarly to the primers used in polymerase chain reaction (PCR), where their specificity directs the DNAzyme to its target.

SELEX Method for DNAzyme Preparation

SELEX, or Systematic Ligand Evolution by Exponential Enrichment, involves a large pool of random nucleic acids. Any nucleic acid that can form a complex with the target molecule is isolated, amplified through PCR, and pooled together to create a new library for subsequent rounds of SELEX selection. Typically, 5 to 15 rounds of screening are conducted, and the final nucleic acids obtained are potential candidates. Instead of rationally designing DNAzymes that utilize known DNAzyme catalytic cores, SELEX identifies novel DNAzymes and catalytic cores.

General Workflow for DNAzyme Preparation

Fig.1 Flow chart of DNAzyme preparation. (CD Formulation)

• Target Sequence Analysis

An in-depth analysis of target RNA or DNA sequences is conducted to evaluate potential targets and their structural features, which helps determine the most effective design strategy.

• DNAzyme Design

The design of specific DNAzyme sequences utilizing bioinformatics tools to ensure optimal catalytic activity and target specificity.

• Synthesis of DNAzymes

Synthesize the desired DNAzyme using either chemical synthesis or enzymatic synthesis based on the designed sequence.

• Structure Verification

Verify the structure and purity of the synthesized DNAzyme using experimental techniques such as gel electrophoresis and conformational analysis to ensure it meets the design requirements.

• Activity Testing

Evaluate the catalytic activity and specificity of DNAzymes in in vitro experiments to assess their cleavage efficiency on target RNA or DNA.

• Optimization Adjustment

Based on the results of the activity test, implement the necessary optimizations and adjustments to enhance its catalytic efficiency and specificity.

Our DNAzyme Synthesis Technology Platforms

CD Formulation's DNAzyme synthesis technology platform provides tailored solutions for DNAzyme creation and optimization. Our integrated approach ensures accurate, reliable and scalable DNAzyme production.

• Solid Phase Synthesis Technology Platform
• Enzymatic Synthesis Technology Platform

Highlights of DNAzyme Synthesis Services

• DNAzymes' ability to target specific RNA or DNA sequences minimizes effects on non-target molecules, leading to fewer side effects.
• DNAzymes efficiently cleave target RNA, making them ideal for therapeutic use.
• DNA synthesis technology enables rapid large-scale production of DNAzymes.
• DNAzymes can be engineered to perform specific biological functions, including gene expression regulation.
• DNAzymes can be engineered for multiple functions, such as targeting, cleavage, and regulation.

Publication Data

CD Formulation offers customized services for DNAzymes, including design, screening, and optimization, through a specialized biotechnology platform. Contact us, and we will help you find innovative solutions.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.