Antibody-siRNA Conjugate Development

Antibody-siRNA conjugate delivery systems can be created by directly conjugating siRNA to various chemical groups within an antibody. Traditional antibody-siRNA conjugates mainly use natural lysine or reduced cysteine residues. CD Formulation offers cost-effective conjugation services and supports large-scale development for global clients.

What are Antibody-siRNA Conjugates?

Antibody-small interfering RNA (siRNA) conjugates (ARCs) combine the specificity of antibodies with siRNA's gene-targeting capabilities. This method uses antibodies for precise targeting and siRNAs for gene silencing, delivering therapeutic payloads to specific cells to inhibit gene expression. ARCs address challenges of inadequate siRNA targeting and limited circulation, offering a promising solution for targeted gene silencing and enhanced therapeutic efficacy.

Fig.1 Synthesis of antibody-siRNA conjugates. (CD Formulation)

Antibody-siRNA Conjugate Development Services

Customized Development of Antibody-siRNA Conjugates

The antibody-siRNA binding site is selected at the 3' end of the S gene, a region typically designed for specific recognition and binding. In antibody-siRNA synthesis services, the biotin-streptavidin system is commonly employed in the coupling reaction. This chemical coupling reaction leverages the high affinity between biotin and streptavidin to achieve efficient linkage of the antibody to the siRNA, ensuring the stability and functional integrity of the bicomponent throughout the synthesis process.

Antibody-siRNA Conjugate Optimization Services

General Workflow for Antibody-siRNA Conjugate Development Services

Fig.2 Flow chart of antibody-siRNA conjugate development. (CD Formulation)

• siRNA Design and Synthesis

Design specific siRNA based on the target sequence, ensuring high-purity synthesis that includes biotinylation modification at the 3' end for subsequent binding.

• Antibody Selection and Biotinylation

We assist customers in selecting suitable antibodies and biotinylating them to ensure efficient binding to streptavidin.

• Antibody-siRNA Complex Construction

Combine biotin-labeled siRNA with antibodies to form stable complexes and optimize binding conditions for better efficiency.

• Quality Control

Remove unbound components using efficient purification techniques, such as gel filtration and ultrafiltration. Conduct quality control using various methods, including SDS-PAGE and mass spectrometry.

Advantages of Our Antibody-siRNA Conjugate Development Services

• Combining siRNA with specific antibodies enables targeted delivery to specific cells or tissues.
• Antibody binding protects siRNA from degradation, enhances stability, and increases its half-life and bioavailability in vivo.
• Antibody-siRNA complexes minimize the immune response compared to traditional viral vectors, reducing the risk of adverse reactions.

Synthesis Platforms

Solid-Phase Synthesis Platforms - Solid-phase synthesis technology for developing antibody-siRNA complexes is an efficient and controlled method for preparing high-purity siRNAs, which can subsequently undergo biotinylation modifications.

Published Data

CD Formulation offers professional antibody-siRNA development services, including siRNA design and synthesis, antibody selection and modification, complex construction, purification, and validation. Our comprehensive approach ensures high-quality products and exceptional experimental results. For more information, please do not hesitate to contact us.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.