Lipid Nanoparticle Development for Nanomedicine

Active pharmaceutical ingredients encapsulated in lipid nanoparticles (LNPs) are protected from enzymatic degradation during the delivery process and are efficiently delivered into cells, where the contents of the carrier particles are released and translated into therapeutic proteins. LNPs therefore have great revolutionary potential in the delivery of oligonucleotide-based therapies and other drugs. CD Formulation utilizes cutting-edge lipid nanoparticle manufacturing technology to offer customers services in developing LNP formulations tailored to their specific requirements, including creating liposomes, solid lipid nanoparticles (SLNs), Nanostructured lipid carriers (NLCs), and lipid-polymer hybrid nanoparticles, etc.

Classification and Advantages of Lipid Nanoparticles

Lipid nanoparticles are spherical vesicles composed of one (monolayer) or multiple (multilamellar) phospholipid bilayers. It is usually composed of four components, namely cationic lipids, helper lipids, cholesterol and pegylated lipids (PEG-lipids). The classes of lipid-based nanoparticles for drug delivery include liposomes, solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), lipid-polymer hybrid nanoparticles, etc.

Fig.1 Structures of different types of lipid-based nanoparticles. (Yining Xu, et al. 2022)

As a drug delivery system, liposomes can encapsulate these drugs and improve their water solubility, reducing the toxicity of drugs to normal tissues and prolonging the residence time of drugs. Many lipid nanoparticle drug formulations have been widely used in many clinical trials as delivery systems for anticancer, anti-inflammatory, antibiotic, antifungal, anesthetic, and other drugs and gene therapies, especially in the field of delivering nucleic acid drugs.

Our Workflow for Lipid Nanoparticle Development

Lipid nanoparticles have the capability to package and deliver various drugs (such as mRNA, siRNA, small molecule drugs) to targeted areas of the body in a controlled manner, which is beneficial for treating a range of diseases. We are dedicated to advancing lipid nanoparticle research and development. We offer thorough, cutting-edge, and prompt solutions to assist you in efficiently creating high-quality lipid nanoparticles for nanomedicines. And our workflows for lipid nanoparticles is as follows.

Fig.2 Our workflow for lipid nanoparticle development. (CD Formulation)

Explore Our Preparation Methods for Lipid Nanoparticles

CD Formulation has developed many different methods to prepare lipid nanoparticles (LNPs) through years of research experience.

Solvent-Based Emulsification Method

This method is to dissolve solid lipids and hydrophobic drugs in water-immiscible organic solvents (such as cyclohexane, toluene, and chloroform), and then disperse them in an aqueous solution to form an oil-in-water emulsion. And this method includes emulsion-solvent evaporation, solvent diffusion, solvent displacement, solvent injection, etc.

Non-solvent Emulsification Method

This method does not use a solvent to dissolve lipids, but uses a non-solvent emulsifier cationic method, which uses molten lipids as the liquid phase to form an oil-in-water emulsion, so it is also called the melt emulsification method.

Nanoprecipitation

Nanoprecipitation, also known as solvent displacement, involves mixing a water-miscible solvent (the so-called organic phase) containing lipids and hydrophobic drugs with an aqueous phase to create lipid nanoparticles.

Microfluidic Approaches

The process of preparing lipid nanoparticles (LNPs) using microfluidic devices is often based on hydrodynamic flow focusing (HFF) designs.

Coacervation Method

To avoid the use of toxic organic solvents and expensive and complex precision equipment, we developed a novel solvent-free method for the preparation of LNPs.

SCF Technology

SCF technology uses supercritical forms of materials to prepare lipid nanoparticles with adjustable solvent power by changing pressure and temperature.

Apart from the unique methods mentioned earlier, we offer the option to customize lipid nanoparticles containing various drugs such as water-insoluble drugs, water-soluble drugs, and RNA.

Our Analytical Capabilities for Lipid Nanoparticles

Accurate characterization of lipid nanoparticles (LNPs) is essential for understanding their synthesis and maintaining their quality. We offer comprehensive characterization services for LNPs to ensure their quality control.

Why Choose Us to Develop Lipid Nanoparticles?

• We hold a strong and elite team of experts. We can promptly respond to your needs for lipid nanoparticle formulation development for nanomedicine, including liposomes, solid lipid nanoparticles, nanostructured lipid carriers, lipid-polymer hybrid nanoparticles, etc.
• Based on our advanced world-class liposome nanoparticle technology platform, we can quickly develop full-process service solutions from formula design, and lipid nanoparticle synthesis, to commercial large-scale production to meet your project needs.
• We also have a long-term focus on the exploration of synthesis technology of lipid nanoparticles, including solvent-based emulsification, non-solvent emulsification, nanoprecipitation, etc.

Published Data

In recent years, the research on lipid nanoparticles as nucleic acid drug delivery carriers has entered a heated stage. As your most loyal partner, CD Formulation has accumulated rich and valuable experience in the development and synthesis of lipid nanoparticles. If you are interested in our services, please feel free to contact us for in-depth discussions.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.