Proliposomes are produced by encapsulating phospholipid solutions into a sugar matrix using methods such as rotary evaporation, spray drying, or fluidized bed coating. Upon hydration, proliposomes transform into functional liposomes, offering improved stability and ease of handling. CD Formulation specializes in leveraging this innovative technique to support researchers and pharmaceutical development projects. Our expertise enables the production of high-quality proliposomes that address challenges associated with traditional water-soluble liposomes, such as aggregation and fusion.

Fig.1 Illustration for Release-controlled curcumin proliposome. (Jia J, et al, 2016)

What is the Proliposome Method for Liposome Preparation?

The thin-film hydration method, commonly referred to as the Bangham method, is named after the British scholar who first identified liposomes, Bangham. This technique represents the most established approach for liposome preparation. The process entails dissolving phospholipids in an organic solvent, followed by evaporating the solvent under reduced pressure to yield a lipid-thin film. Subsequently, a suitable aqueous medium is introduced. At the phase transition temperature of the phospholipids, this lipid thin film hydrates and detaches, self-assembling into closed spherical structures known as liposomes. This method is straightforward and easily executed, achieving nearly 100% encapsulation efficiency for most lipophilic drugs. However, it tends to produce liposomes with a heterogeneous size distribution; thus, necessitating size control measures such as polycarbonate membrane extrusion or homogenization techniques. Furthermore, maintaining sterile conditions throughout the procedure is essential to ensure product sterility.

Our Proliposome Preparation Services

Screening Methods for Proliposome Customization

Due to diverse factors such as vesicle size, size distribution, encapsulation capacity, and retention of contents, it is necessary to select the appropriate method. We screen drugs based on their physicochemical properties, type of phospholipid required, target particle size range, and preparation method.

Proliposome Characterization

Primarily for the characterization of services including, but not limited to, particle size, zeta potential, encapsulation efficiency, drug loading, etc.

Hydration Study and Vesicle Formation

It is important to determine the formation of liposomal vesicles following hydration of the proliposomal formulation in vitro. We confirm the formation of vesicles with light microscopy: The liposome suspension should be applied onto a glass slide and air-dried at room temperature. Subsequently, the resulting dry film of the liposome suspension should be examined for vesicle formation.

In Vitro Release from Proliposomes

We conducted in vitro drug release studies of proliposomes using various techniques, such as USP dissolution apparatus Type I, Franz diffusion cells, dialysis tubes, reverse dialysis, glass paper diffusion membranes, transparent diffusion cells, and spectral molecular porous membrane tubes.

Our Capacities for Proliposome Method for Liposome Preparation

Why Choose CD Formulation?

Advanced Proliposome Techniques

• Proficient in liposome thin-film hydration (TFH) preparation technique
• Formulation development capability
• Process optimization capability
• Size control capability

Powerful Proliposome Analysis Platforms

• Advanced platform supports the development and customization of multiple proliposomes, based on a various preparation processes and methods.
• Comprehensive analysis service. From physical and chemical properties, in vitro release studies, to in vivo evaluation, we guarantee to provide our customers with highly stable, effective, and less toxic side effects of the proliposomes.

A Great and experienced Team

• Successfully provided vital support to numerous corporations and academic institutions, enabling them to delve deeply into liposome thin-film hydration (TFH) preparation techniques.
• Our teams consist of many scientists with multi-disciplinary backgrounds who are well-versed in the development and evaluation of multiple proliposomes.

Published Data

CD Formulation provides customized proliposome preparation services to facilitate innovative lipid-based drug delivery systems. By addressing the inherent limitations of water-soluble liposomes, our solutions ensure enhanced formulation stability and efficiency, supporting a wide range of pharmaceutical R&D applications. Contact us for expert assistance with your proliposome preparation needs.