Integrating flow cytometry and labeling techniques offers a promising platform for disease diagnosis, treatment, and studies on various biological processes. CD Formulation is committed to the development of diverse liposome-based labeling products for flow cytometry, utilizing state-of-the-art technology and expertise.

What are Labeled Liposomes for Flow Cytometry?

Metabolic research has long been a pivotal focus within life sciences. Flow cytometry serves as a high-throughput and highly sensitive cell analysis technique that is progressively gaining widespread adoption in metabolic studies. It holds substantial relevance in areas such as infectious diseases, healthcare management, autoimmune disorders, and hematologic conditions including tumors and primary immunodeficiencies. Experiments utilizing flow cytometry techniques for tasks like cytokine detection and immune typing impose stringent requirements on diverse fluorescently labeled antibodies and fluorophore selection due to their direct impact on detection efficacy. Fluorescent-labeled liposome particles present a novel avenue for cellular metabolic investigations.

Fig.1 Quantitative assessment of the intracellular uptake of fluorescent liposomes. (Lee, Sooyeon, et al., 2013)

Custom Labeled Liposome Service for Flow Cytometry

The technology of flow cytometry facilitates rapid quantitative analysis and sorting of individual cells or other biological particles (such as microspheres, bacteria, and miniature model organisms) in a liquid stream. The utilization of liposomes in flow cytometry primarily encompasses cell transfection and liposome probes.

Liposomal formulations for cellular transfection

Liposomes serve as essential tools for in vivo and in vitro delivery, primarily utilized to encapsulate synthetic cationic lipids and DNA through fusion for cellular entry. Compared to alternative methods, liposomes for DNA delivery into diverse eukaryotic cells offer superior efficiency and reproducibility. We assist our clients in developing gene therapy products based on liposomes by providing fluorescently labeled liposome products.

Fluorescently labeled liposome customization

Fluorescein is categorized into four groups based on the excitation light wavelength, specifically 375nm, 405nm, 488nm, and 633nm. Among these, commonly used fluoresceins include those with excitation wavelengths of 488nm and 633nm, such as FITC, PE, APC, PI, etc. We are able to tailor liposome probes for flow cytometry to accommodate various fluorescence excitation wavelengths. We also provide quantum dots for multicolor labeling options, enabling multiplexed flow cytometry experiments.

Our Platforms and Techniques

Why Choose CD Formulation?

• Outstanding techniques for labeling liposomes. We provide customized liposome probes and a diverse range of fluorescent molecules to support multi-channel flow cytometry experiments, as well as offer quantum dots for multi-color labeling options.
• The proficient teams. The technical team comprises seasoned and highly skilled experts proficient in liposome labeling technology for flow cytometry, with diverse multidisciplinary backgrounds and extensive field knowledge.
• Our high specificity labeling liposomes. Custom labeling ensures precise visualization of liposome-cell interactions.

Published Data

As a leading company in nanoparticle development, CD Formulation is dedicated to providing excellent labeling of liposome products for flow cytometry. Please do not hesitate to contact us if you require any assistance.