Naked DNA Development Service

Naked DNA-mediated gene therapy refers to the delivery of naked DNA (without viral vectors) carrying target genes directly to the target cells to realize the transcription and expression of genes with the help of the cell's own genetic expression mechanism, so as to achieve the purpose of preventing or treating diseases. This method does not need to rely on viral vectors, so it is highly safe and simple. Naked DNA is injected directly into target cells by intramuscular injection, intradermal injection or intravenous injection, and the expression of target genes is accomplished in the cells, thus producing therapeutic effects. This approach can be applied to gene therapy for a variety of diseases, such as hereditary diseases, tumors, infectious diseases, and so on.

CD Formulation is a leading global biotechnology service provider, with many years of professional and rich experience, we can provide you with a full range of gene therapy vector delivery system development services. We can provide customized gene therapy non-viral vector development services according to your project requirements, and our professional knowledge and advanced technology can effectively help you promote the research and development of gene therapy.

Advantages of Naked DNA Development

• Low immunogenicity. Naked DNA itself is of low immunogenicity and will not trigger a strong immune response. It can be administered repeatedly without producing neutralizing antibodies.
• High safety. Compared with viral vector gene therapy, naked DNA therapy does not have the risk of viral infection and avoids potential safety hazards such as viral replication and immune response, thus it has higher safety.
• Simple preparation. Naked DNA does not require complex preparation of viral vectors, the preparation process is relatively simple and fast, low cost, and easy to mass production, which makes gene therapy more economically feasible.
• Strong targeting. By regulating the DNA sequence and the route of administration, it can realize efficient target transfection of specific cells or tissues.
• Long-lasting gene expression. Naked DNA can be continuously transcribed and expressed in the cell nucleus, which can realize long-term therapeutic effects.

What We Offer to Develop Naked DNA?

Screening of target genes and vector construction

First of all, we need to determine the therapeutic target and select suitable therapeutic genes, such as corrective genes, suicide genes or immunomodulatory genes, etc. Then we will clone the target gene into the appropriate plasmid vector and construct the expression vector. Then, the target gene should be cloned into the appropriate plasmid vector and the expression vector should be constructed.

Purification and preparation of naked DNA

Plasmid amplification, extraction, purification and other technical means are used to obtain high-purity naked DNA samples. The concentration, purity and structural integrity of naked DNA should be strictly controlled to ensure the quality.

Optimization of route of administration and dosage

According to the different indications, the appropriate route of administration, such as intramuscular injection, intradermal injection, intravenous injection, etc., should be selected. At the same time, the dosage should be optimized to ensure effective and safe treatment.

Transfection and gene expression in target cells

After the naked DNA enters the target cells, it can realize the expression of the target gene through the transcription and translation mechanism in the cells. The transfection efficiency can be evaluated by monitoring the gene expression level and therapeutic effect.

Applications of Our Naked DNA Development Service

• Hereditary diseases. Naked DNA can be involved in immunoregulation, apoptosis, or other processes that promote tumor cell apoptosis or stimulate the immune system to prevent tumor growth.
• Tumor diseases. Naked DNA vectors express genes involved in immunoregulation, apoptosis, or other processes that promote tumor cell apoptosis or stimulate the immune system to prevent tumor growth.
• Infectious diseases. To prevent and treat infectious diseases including AIDS, malaria, tuberculosis, and others, the body is stimulated to create an immune response against pathogens through the production of vaccine antigen genes or immunoregulatory genes.
• Other diseases. Naked DNA gene therapy can also be applied to the treatment of cardiovascular diseases, neurological diseases, metabolic diseases and so on. For example, it can be used to treat vascular diseases by expressing the vascular growth factor gene and to treat neurological diseases by expressing the nerve growth factor gene.

Our Platforms for Naked DNA Development

Highlights of Our Naked DNA Development Service

• Customized solutions. We provide customized solutions for non-viral vector development based on our customers' research goals. This technical service ensures that naked DNA is optimized for your specific application.
• Professional research team. Our team has deep knowledge in the field of gene delivery, which enables us to provide the most effective and reliable technical methods to deliver naked DNA to target cells.
• Innovative solutions. We are always at the forefront of gene therapy development. We provide innovative naked DNA development technologies to increase the possibility of successful gene expression.
• Efficiency and safety. We continue to simplify the development process to make naked DNA more efficient and safer in gene delivery.

Published Data

CD Formulation has many years of research service experience in gene therapy. In naked DNA development, we have established core technologies including target gene design and screening, plasmid vector construction, production processes, quality standards, and so on. Relying on our existing technology platform, we are committed to promoting the research and application of naked DNA delivery system in gene therapy. If you are interested in us, please feel free to contact us.