Adenovirus Vector Development Service

Adenovirus is a large (36 kb) double-stranded non-enveloped DNA virus. It enters cells through receptor-mediated endocytosis, and then the adenoviral genome is transferred to the nucleus, where it remains extrachromosomal and does not integrate into the host cell genome. Adenoviruses are characterized by infection of dividing and non-dividing cells, high immunogenicity, and large genetic capacity. Adenovirus vector, as a common viral vector, plays an important role in gene therapy. To date, we have developed various methods for constructing recombinant adenoviruses. CD Formulation provides advanced adenoviral vector construction and development technology services, which greatly facilitates the research and application of adenoviral vector development in gene therapy.

Advantages of Adenovirus Vector

• Wide host range, low human pathogenicity. Adenoviral vector systems are widely used for the expression of human and non-human proteins. Adenoviruses can infect a wide range of mammalian cells and can therefore be used to express recombinant proteins in most mammalian cells and tissues.
• Proliferation can be carried out efficiently with high titers. The adenovirus system produces 1010 to 1011 VP/ml, and up to 1013 VP/ml when concentrated, a feature that makes it very suitable for gene therapy.
• Homologous to human genes. Adenoviral vector systems generally apply human viruses as vectors and use human cells as hosts, thus providing an ideal environment for accurate post-translational processing and proper folding of human proteins.
• No integration into chromosomes, and no insertion mutagenicity. Adenoviruses, on the other hand, do not integrate into chromosomes in almost all known cells except oocytes and therefore do not interfere with other host genes.

What We Offer to Develop Adenovirus Vectors?

Gene retrieval and construction of adenovirus shuttle plasmid

We will obtain the CDS region information of the target gene and design primers for the gene of interest, then we will retrieve the gene from the library and clone it into the adenovirus shuttle vector (Pshuttle-CMV). If the gene to be cloned is a mutant, the gene will be cloned into the shuttle vector, and then several point mutations will be performed until the desired mutant is obtained.

Homologous recombination of adenoviral backbone plasmids

After linearization of the shuttle plasmid carrying the target gene fragment, it is co-transfected with the adenoviral backbone plasmid into specific E.coli for homologous recombination. Since the adenoviral backbone plasmid is ampicillin resistant, and after homologous recombination with the shuttle plasmid, ampicillin resistance is lost, and kanamycin resistance is expressed at the same time, therefore, through the change of resistance, we can screen out the adenoviral vector backbone recombinants, and then select the recombinants for enzyme digestion, and then select the correct clones for enzyme digestion. The recombinants were picked for enzyme digestion and identification, and the clones with correct enzyme digestion and identification were selected for the packaging of the adenoviral vector in the next step.

Packaging and amplification of adenoviral vectors

Adenoviral vectors can be packaged by transfecting the screened recombinant adenovirus into specific packaging cells, and the viral particles can be enriched by multiplicative amplification.

Fig.1 Our development process of adenoviral vectors. (CD Formulation)

Nowadays, the adenovirus system has been widely used in gene overexpression of various cell lines, RNA interference, miRNA research, and live animal experiments. The adenoviral vector development we offer plays an important role in helping researchers advance gene therapy research. If you need us to provide you with services related to the development of adenoviral vectors for gene therapy, you can contact us at any time, and you can also check the real-time progress of the project at any time when it is determined to be carried out. When the experiment is completed, we will notify the customer to view the experiment report in time.

Applications of Our Adenovirus Vector Development Services

• Treatment of neurologic diseases. The application of adenoviral vectors to neurological diseases is an important application area in gene therapy. For example, in the treatment of Parkinson's disease, the use of adenoviral vectors to implant habitual motor neurons into brain cells can effectively treat the pathogenesis of the disease and reduce the motor symptoms of Parkinson's patients.
• Gene vaccines. In vaccine research, Adenoviral vectors are often used as delivery vectors for antigens, where protective antigen genes are inserted into the Ad genome, and a recombinant Ad is generated as a potential candidate vaccine. After immunization, the recombinant Ad will activate the body's immune system, resulting in massive production of protective antibodies.
• Anti-cancer therapy. Adenoviral vectors can be used to cause growth arrest or apoptosis of these tumor cells, and can also be used to achieve tumor cell killing by expressing the translocases of certain precursor drugs. In addition, adenoviral vectors can be used to stimulate anti-tumor immune responses in patients by delivering expression of immunomodulatory genes.

Our Platforms for Adenovirus Vector Development

Highlights of Our Adenovirus Vector Development Service

• Efficient development process. We have a systematized and efficient adenoviral vector development process that allows to help our customers save a lot of time needed for gene therapy development.
• Customized development service. We can provide customized adenovirus development services in gene therapy to best meet our clients' research needs.
• Rich experience. We have extensive experience in selecting appropriate coat proteins, optimizing gene expression components, optimizing structures and assembly processes, and optimizing purification and production processes.

Published Data

CD Formulation is committed to gene therapy formulation development. With the support of an advanced technology platform and a professional R&D team, we continue to overcome difficulties, explore and innovate, and provide effective technical support for viral gene delivery systems development service. If you are interested in us, please feel free to contact us.