Adeno-Associated Virus Capsid Protein Identification

Capsid protein plays a crucial role in gene therapy. They are major components of viral vectors such as adeno-associated virus (AAV) and determine the packaging efficiency, delivery properties, immunogenicity, and tissue specificity of viral particles. The analysis of AAV capsid protein is critical for optimizing viral vector design and improving the safety and efficacy of gene therapy drugs.

CD Formulation aims to provide comprehensive services for developing gene therapy agents, especially in identifying AAV capsid protein. Our reliance on a range of cutting-edge technology enables us to evaluate the caliber of AAV preparations, guaranteeing the security and effectiveness of gene therapy medications.

Why Identify AAV Capsid Protein?

• Ensure the quality of viral vectors. AAV capsid protein is a major component of viral particles, which determines the packaging efficiency and delivery properties of AAV. Rapid and efficient analysis of AAV capsid protein can optimize the design of viral vectors and improve the safety and efficacy of gene therapy drugs.
• Promoting gene therapy product development. The identification of AAV capsid protein is an important step in the development and commercialization of gene therapy products. We analyze AAV capsid proteins for complete protein mass counts to facilitate the development of gene therapy products.
• Improving gene therapy targeting and transduction efficiency. By modifying AAV capsid protein, the targeting and transduction efficiency of gene therapy can be improved.
• Reduced immunogenicity. Modification of AAV capsid protein can reduce its immunogenicity and reduce the effect of pre-existing immunity on gene therapy effect in vivo.
• Improvement of productivity. AAV capsid protein has a great impact on the yield of rAAV. By optimizing the structure of the coat protein, the yield of rAAV can be increased and the production cost can be reduced.

Our Services for AAV Capsid Protein Identification

Protein concentration and stoichiometric ratio analysis

The concentration of VP1, VP2 and VP3 proteins in the AAV coat and the molar ratios between them, which are critical for predicting the infectivity and stability of AAV vectors, were determined by techniques such as Simple Western, HPLC, SEC-MALS, etc.

Protein purity and integrity analysis

SDS-PAGE, western blot and other methods were used to detect the purity and integrity of AAV capsid protein and assess the quality of AAV preparation

Serotype identification

Identify serotypes of structural protein sequences by liquid mass spectrometry platform to ensure the correct serotype of AAV used.

Empty capsid detection

Techniques such as AUC (Analytical Ultracentrifugation) are utilized to quantify the amount of DNA in the AAV capsid and to assess the percentage of empty capsids, which is critical to the safety and efficacy of AAV products.

Post-translational modification analysis

Mass spectrometry (MS) is used to identify post-translational modifications, such as acetylation and phosphorylation, of AAV capsid proteins, which are important to help researchers understand the biological properties of virus particles.

Analysis of capsid protein variants

LC-MS allows the identification of variants of AAV capsid protein, including different forms of capsid protein due to post-translational modifications or proteolysis.

Biological activity analysis

We assessed the biological activity of AAV capsid protein by evaluating the titer of infection and the expression of target proteins, which are essential for demonstrating the effectiveness of AAV as a gene therapy vector.

Fig.2 Adeno-associated virus capsid protein analysis. (CD Formulation)

Our Technologies for AAV Capsid Protein Identification

Highlights of Our AAV Capsid Protein Identification

• Standardized operation process. We have established standardized experimental procedures and operation guidelines to ensure consistency and reproducibility of each experimental operation.
• Use of advanced analytical techniques. We use high-sensitivity and high-resolution analytical techniques to improve the accuracy of detection.
• Professional staff training. We regularly provide technical and theoretical training to our laboratory personnel to ensure that they have a deep understanding of the identification techniques of AAV coat protein and can operate skillfully.
• Data management. We have established accurate, state-of-the-art data recording and analysis systems to ensure that we provide data integrity and traceability.

Published Data

CD Formulation is committed to the development of gene therapy formulations. We are customer-oriented and continue to provide our customers with professional and comprehensive technical support services. If you are interested in us, please feel free to contact us.