Adeno-Associated Viral Vector Development Service

Adeno-associated virus (AAV) vectors are pivotal in the field of gene therapy and are one of the candidate gene therapy vectors due to their low infectivity, low immune response, and ability to transduce multiple tissues. Utilizing the fundamentals of molecular biology and laboratory techniques, CD Formulation offers a wide range of AAV vector development services for basic research.

Advantages of AAV Vector Development

• High safety. Wild-type AAV has never been found to be pathogenic to humans, and the recombinant AAV genome sequence removes most of the wild-type AAV genome elements, which further ensures safety.
• Low immunogenicity. When AAV infects muscle, brain, eye, and other tissues with localized large doses, few infected cells are cleared by the immune system afterward, which is extremely helpful for animal experiments.
• Wide host range. Almost all cells in the division and quiescent phase can be infected with AAV.
• Stable and long-lasting expression. The episome can be formed in the host cell and exist in the cell nucleus, and it can be continuously expressed for more than 5 months in the tissues where cell division is not vigorous.
• Strong diffusivity. rAAV has much higher diffusivity than AdVs and LVs and can penetrate the blood-brain barrier, which makes it the most ideal tool for neuronal and glial cell infections.

What We Offer to Develop Adeno-Associated Viral Vectors?

Selection of vector backbone

Our commonly used vector backbones include pAAV, pHelper pRC, etc. Among them, pAAV is responsible for carrying the target gene, pHelper contains the auxiliary genes required for AAV, and pRC is used for the replication and packaging of AAV.

Cloning of target gene

We insert the target gene into the pAAV vector by usually choosing a suitable restriction endonuclease to cleave the vector and the target gene, and then ligase is used to connect the target gene with the vector, and finally, the recombinant vector containing the target gene is obtained after transformation and screening.

Adding auxiliary genes

This step involves the co-transfection of auxiliary genes in pHelper with the recombinant vector into host cells to provide the auxiliary functions required for AAV replication and packaging.

Production and purification of AAV

Finally, we will culture the transfected host cells, and after a large amount of AAV is expressed, the AAV virus particles will be extracted from the cells by ultracentrifugation and other methods, and purified by filtration, centrifugation, concentration and other steps.

Our AAV vectors can be used for gene therapy studies (both in vivo and in vitro) for a wide range of diseases, and they are also widely used as characterization vectors for gene function studies, disease modeling, and the preparation of knockout mice. If you need technical support for AAV vector development in gene therapy, please feel free to contact us, we can provide one-stop AAV vector development service for you.

Fig.1 Our development process of AAV vectors. (CD Formulation)

Applications of Our Adeno-Associated Viral Vector Development Service

• Adenoviral vectors as gene vaccines. Recombinant adenoviral vectors can be used to create various types of cancer vaccines by expressing tumor-associated antigens or by facilitating the process of tumor lysis. Antigens currently used to create cancer vaccines include, for example, the prostate cancer antigen PSA, the pancreatic cancer antigen CEA, and others.
• Adenoviral vectors in antitumor therapy. Since the p53 oncogenic pathway is dysfunctional in many tumor cells, adenoviral vectors can be used to re-express p53 to cause growth arrest or apoptosis in these tumor cells. Selectively replicating adenoviral vectors can specifically kill tumor cells through tumor lysis.
• Adenoviral vectors deliver a cellular "Suicide Gene". In addition to expressing the oncogene p53, adenoviral vectors can also kill tumor cells by expressing the transforming enzymes of certain precursor drugs.
• Adenoviral vectors deliver immunomodulatory genes. Adenoviral vectors can also be used to stimulate anti-tumor immune responses in patients by delivering and expressing immunomodulatory genes. For example, adenoviral vectors carrying interferon IFN-β or IFN-α-2b genes can be used to treat malignant pleural mesothelioma with a strong safety profile.

Our Platforms for Adeno-Associated Viral Vector Development

Highlights of Our Adeno-Associated Viral Vector Development Service

• Custom development services. We offer customized development approaches to best meet the needs of investigators in gene therapy viral vector development.
• Quality assurance. Our professional research team has many years of development experience in gene therapy development, and our company performs strict quality control at every step from vector design to viral vector purification.
• One-stop development services. We offer a full range of AAV vector development services that can maximize the efficiency of our clients' research.

Published Data

CD Formulation is an industry leader in the development of gene therapy formulations, and we have an experienced team of specialists and years of experience in gene therapy formulation development services. We have proven ourselves to be trustworthy with our services and products. If you are interested in us, please feel free to contact us.