Western Blotting (WB) Detection Service

Western blotting, also known as immunoblotting, it is a technology widely used in the fields of molecular biology, biochemistry, immunology and other fields. It is a technology that uses the specific binding of antibodies to specific proteins in tissue or cell samples to achieve protein identification and expression analysis based on the position and intensity of the bands. This method is a new immunobiochemical technology developed based on gel electrophoresis and solid-phase immunoassay technology. Western blotting has become a routine technique for protein analysis due to its high resolution of SDS-PAGE and high specificity and sensitivity of solid-phase immunoassay. Western blotting is used to identify a certain protein and can perform qualitative and semi-quantitative analysis of the protein. Combined with chemiluminescence detection, the expression levels of the same protein in multiple samples can be compared simultaneously. CD Formulation can provide high-quality western blotting detection services.

The Principle of Western Blotting (WB)

Western Blotting uses SDS-PAGE electrophoresis to separate protein mixtures. After the proteins separated by SDS-PAGE are transferred to a solid support (NC membrane, PVDF membrane, etc.), the solid support Adsorb proteins in the form of non-covalent bonds, and can keep the antigenic properties of the protein or peptide on the membrane unchanged. The protein or peptide on the solid phase carrier is used as the antigen, and an immune reaction occurs with its corresponding antibody. The primary antibody is then combined with the enzyme-labeled The secondary antibody reacts and is developed with a substrate to detect specific protein expression levels.

The Advantages of Western Blotting (WB)

1. Multiple target proteins can be detected simultaneously;
2. Can distinguish proteins with similar molecular weights;
3. Can be quantified accurately;
4. Low background, high signal-to-noise ratio;
5. Wider linear range, suitable for detecting low-abundance proteins.

CD Formulation's Service Advantages

1. CD Formulation has advanced testing instruments and can provide high-throughput antibody testing services;
2. CD Formulation has rich detection experience and special skills in sample processing, transfer and antibody dilution ratio;

CD Formulation has a large product library support, including a variety of cell and tissue lysates, and a variety of antibodies for WB applications.

CD Formulation's immunoassay team has extensive experience in western blotting (WB) detection service. Our team of highly skilled scientists and engineers is committed to providing you with the highest quality service to meet your specific research and needs. Thank you for choosing our company's western blotting (WB) detection service. If you have any related needs, please contact our staff and we will answer and serve you immediately.