Recombinant Antibody Preparation Services

Recombinant antibody, also known as genetically engineered antibody, refers to the antibody obtained by transforming and reconstructing the corresponding gene sequence of the antibody according to the needs through DNA recombination technology, and constructing the plasmid, and then transfecting/transforming the constructed plasmid into a suitable host cell for expression through protein exogenous expression technology. Recombinant antibody can solve the rejection reaction caused by animal-derived antibody well, realize the humanization of antibody, and improve the efficacy of antibody. Compared with traditional monoclonal antibodies and polyclonal antibodies, recombinant antibodies have many unique advantages and are widely used in scientific research, immunodiagnosis and therapeutic antibodies. CD Formulation has extensive experience in the field of recombinant antibodies, and we are capable of providing you with the production of multiple forms of recombinant antibodies, including chimeric antibodies, humanized antibodies, fully humanized antibodies, small molecule antibodies, and bispecific antibodies.

Advantages of Recombinant Antibody

• High consistency and high repeatability: The gene sequence of the recombinant antibody is determined, so the difference between antibody batches is small and the repeatability is good.
• Short cycle time: The production cycle of recombinant antibodies is 6 to 8 weeks, while the preparation of hybridoma antibodies takes several months.
• Easy to transform: Recombinant antibodies can be recombinantly transformed, including expression of antibody fragments, isotype substitution, chimeric antibodies, antibody humanization, etc.
• Large-scale production: Recombinant antibodies enable large-scale preparation of antibodies without the use of animals.

Our Services

CD Formulation can provide the preparation of these antibodies: chimeric antibodies, humanized antibodies, fully humanized antibodies, small molecule antibodies, and bispecific antibodies.

Chimeric antibodies combine the variable region of an animal-derived antibody with the constant region of a human-derived antibody through genetic engineering technology. The most common type is a human-mouse chimeric antibody, in which the variable region of the antibody is derived from a mouse antibody and the constant region is derived from a human antibody. Such antibodies not only maintain the specificity and affinity of the original monoclonal antibodies, but also greatly reduce the immunogenicity in the human body.

Humanized antibodies are produced by replacing the CDRs (complementarity determining regions) of human antibodies with the CDRs of mouse monoclonal antibodies using CDR grafting genes. It largely retains the affinity and specificity of the maternal mouse monoclonal antibody. Its humanization ratio can reach 80%-90%, which greatly reduces the immune side effects of heterologous antibodies on the human body.

Fully humanized antibodies refer to antibodies whose genetic sequences are all derived from humans. Mainly through antibody display technology and transgenic mouse technology, all human antibody-coding genes are transferred into genetically engineered antibody gene-deficient animals. After the animals are immunized, fully humanized antibodies are directly produced.

Small molecule antibodies are antibodies with smaller molecular weights and are generally part of a complete Ig. The existing small molecule antibodies include Fab, Fv, scFv, SdAb, microbodies, and nanobodies.

Bispecific antibodies are antibodies with two specific antigen-binding sites that can bind to two antigens at the same time, such as target cells (cancer cells) and effector cells (T cells), and directionally mediate the killing effect of effector cells on target cells.

Recombinant Antibody Preparation Process

At CD Formulation, Tthe preparation process of recombinant antibody is as follows:

CD Formulation can prepare antibodies according to customer requirements, with fast delivery and favorable prices. Thank you for choosing CD Formulation's recombinant antibody preparation services. If you have any relevant questions, please contact our staff and we will answer and serve you as soon as possible.