Phage Antibody Library Technology

Phage display technology is the use of genetic engineering technology to connect the antibody gene to the phage and display it on the phage surface in the form of fusion protein. Through binding with the target protein, the phage display antibody screening is completed. In 1985, Smith GP used genetic engineering to insert foreign genes into the genome of Filamentous bacteriophage (fd), so that the peptides encoded by the target genes were displayed in the form of fusion proteins, thus creating phage display technology. After the creation of phage display technology, it has become an important research method in biological research, fundamentally changing the traditional monoclonal antibody preparation process (hybridoma technology), and is widely used in the establishment of antigen antibody library and drug design. Based on our expertise in the construction and screening of phage display antibody libraries, CD Formulation provides customized antibody services for our customers, including mouse monoclonal antibodies, rabbit monoclonal antibodies and human antibodies of various species.

Advantages of Phage Antibody Libraries

The construction of antibody libraries using phage display technology eliminates the cell fusion step, avoids the cumbersome procedures of repeated subcloning due to hybridoma instability, and greatly increases the library capacity from thousands of hybridoma clones to 10⁶. Phage display technology can directly obtain antibody genes, which facilitates the further construction of various genetically engineered antibodies. It can also be used for some antibodies that are difficult to prepare, such as weak immunogens, toxic antigens, etc., as well as humanized antibodies. Because phage display technology has a short cycle and adds value in bacteria, it is suitable for large-scale industrial production of antibodies. The following table is a comparison of phage display technology and hybridoma technology.

Table 1. Comparison of different antibody preparation methods

Screening Process of Phage Display Technology