Other Lipids Customization

CD Formulation provides comprehensive lipid customization services to support the engineering of targeted lipid nanoparticles (LNPs) for nucleic acid and small molecule delivery. In addition to developing cationic and ionizable lipids optimized for key biophysical properties and transfection activity, our platform enables precision design of tailored helper lipids.

Why are Helper Lipids Needed for LNPs Development?

• They help facilitate the efficient production of nanoparticles with small, uniform sizes through their ability to promote curvature when assembled with ionizable lipids.
• Helper lipids also improve nucleic acid encapsulation and stability during circulation.
• They further aid in the endosomal escape of cargo through their ability to fluidize and destabilize endosomal membranes.
• The addition of optimized helper lipids leads to LNPs with enhanced transfection activity, pharmacokinetics and tolerability.

Schematic diagram of the composition of lipid-based nucleic acid delivery systems (Hald Albertsen C.; et al. 2022)

What Can We Do for Lipids Customization?

• Custom Lipid Synthesis

Through both rational design and high-throughput methods, we can incorporate a wide array of modifications to tune lipid properties. This includes alterations to the head group such as varying chain length and ionization potential. Tail group customizations include modifications to chain length, degree of saturation, branching patterns and functional attachments. We also offer head group and tail group conjugation options such as linking lipids to targeted ligands or triggers.

• Custom Lipid Formulation

A key part of developing effective nucleic acid delivery systems is optimizing the formulation of customized lipids. At CD Formulation we offer a comprehensive custom formulation service. Leveraging our deep understanding of lipid-nucleic acid interactions, we help customers tailor lipid composition and ratios in formulations to customize lipid-based liposomes, nanoparticles, and lipid-drug conjugates.

• Custom Lipid Process/Packaging

Our manufacturing scientists can develop scalable purification and isolation protocols specific to each lipid's physicochemical properties. We have experience with multiple modes of processing and packaging including solution-phase processing, thin-film hydration, microfluidic production, and lyophilization. This allows us to format lipids and formulations as liquids, powders or free-flowing particles optimized for stability, dosing requirements and route of administration.

Types of Lipids We Can Customize

Our Lipid Analysis Services

• HPLC enables the separation and quantification of individual lipid components from complex mixtures.
• MS methods like ESI and MALDI facilitate lipid identification and structural elucidation.
• NMR spectroscopy furnishes insights into lipid structure, composition, and dynamics.
• TLC aids in separation and identification based on lipid mobility.
• Lipidomic analysis involves the comprehensive profiling and quantification of lipid species within a sample, offering a global view of lipid composition and changes.

Why Choose Our Lipids Customization Services?

• We use computational tools to rationally design and synthesize novel lipid structures with targeted properties.
• High-throughput screening systems with automated assays enable rapid evaluation of large libraries.
• Comprehensive analytical characterization techniques are used to gain insights into structure-activity relationships.
• Our formulation scientists can convert custom lipids into optimized nanoparticles and other delivery systems.
• Our comprehensive in vitro/in vivo disease-relevant models help customers evaluate lipid efficacy while characterizing mechanisms of action.

CD Formulation's integrated platform and multidisciplinary expertise help customers accelerate lipid programs from discovery to commercialization. If you would like to learn more about our lipid customization services, please contact us!