Nucleic Acid Purity Analysis

CD Formulation values our customers' feedback and suggestions, and has many years of experience in nucleic acid purity analysis and is always looking for ways to improve our testing services.

Why to Perform Nucleic Acid Purity Analysis?

Residual chemical contamination from nucleic acids extraction procedures may result in an overestimation of the nucleic acid concentration and/or negatively influence downstream analysis. And nucleic acid purity is a very important quality characteristic for the application of nucleic acid bio-pharmaceuticals such as DNA or RNA. Therefore, pure and intact DNA is critical for many downstream assays.

Our Method for Nucleic Acid Purity Analysis

CD Formulation can provide the following three methods for one-stop service for nucleic acid purity analysis.

• UV Spectrophotometry

UV spectrophotometry is the most widely used method to determine the concentration and purity of nucleic acids. To evaluate DNA purity, measure absorbance from 230 nm to 320 nm to detect other possible contaminants. The most common purity calculation is the ratio of the absorbance at 260 nm divided by the reading at 280 nm. Good-quality DNA will have an A₂₆₀/A₂₈₀ ratio of 1.7–2.0. A reading of 1.6 does not render the DNA unsuitable for any application, but lower ratios indicate more contaminants are present. The ratio can be calculated after correcting for turbidity (absorbance at 320 nm).

DNA purity (A₂₆₀/A₂₈₀) = (A₂₆₀ reading – A₃₂₀ reading) ÷ (A₂₈₀ reading – A₃₂₀ reading)

• Fluorescence Method

The widespread availability of fluorometers and fluorescent DNA-binding dyes makes fluorescence measurement another popular option for determining of DNA purity. Fluorescence measurements are set at excitation and emission values that vary depending on the dye chosen. The concentration of unknown samples is calculated based on comparison to a standard curve generated from samples of known DNA purity.

• Agarose Gel Electrophoresis

Agarose gel electrophoresis is an electrophoresis method that uses agarose as a supporting medium. Agarose gel has a network structure. When it is placed in an electric field, negatively charged nucleic acid molecules will migrate toward the positive electrode.

Our Workflow for Nucleic Acid Purity Analysis

You only need three steps to easily solve your requirements.

Advantages of Our Services

• Advanced technology platforms: We are equipped with multiple technology platforms to accurately determine nucleic acid purity.
• Professional technical team: We have an experienced technical team that can select appropriate methods for nucleic acid purity analysis based on customer needs to ensure the accuracy and reliability of the results.
• Super clear service process: We have a very clear service process to guide customers quickly and clearly on the progress of the project.

How to Contact us?

If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.