Nanocapsule Development for Peptide & Protein Delivery

CD Formulation, a pioneering biologics company dedicated to advancing drug delivery technologies. Our expertise lies in the development of nanocapsules, specialized delivery systems that optimize the transportation of peptides, proteins, and antibodies. As leaders in pharmaceutical nanotechnology, we collaborate with biotech and pharmaceutical partners to create scalable manufacturing processes and customized formulations.

Applications of Nanocapsules in Drug Delivery

Nanocapsules, ranging from 10 nm to 1000 nm, consist of a core where drugs are placed and surrounded by a polymer membrane. They have attracted interest due to their protective coating, delaying the release of active ingredients. Nanocapsules are obtained through interfacial polymerization or nano-deposition methods. Various techniques can evaluate their particle size and size distribution. Nanocapsules have diverse applications in fields such as agrochemicals, genetic engineering, cosmetics, drug delivery, and more. They hold promise for targeted drug release and improved therapies. With their small size and high reproducibility, nanocapsules offer opportunities for innovative research and development in delivering bioactive molecules.

How Do We Prepare Nanocapsules?

• Nanoprecipitation

Typically, we combine the oil, polymer and active compound dissolved in an organic solvent to form the organic phase, which is then slowly added to the aqueous phase (in most cases water and selected surfactants) with moderate stirring. The formation of nanocapsules is the result of polymer precipitation at the water-oil interface during spontaneous emulsification and phase diffusion of oil droplets.

• Emulsification-Based Methods

Emulsification is a core technique we leverage to produce nanocapsules with precise control over morphology and delivery properties. Our expertise in emulsion-based processes includes:

Emulsion diffusion/evaporation to emulsify an organic polymer solution into an aqueous phase, then eliminate the organic solvent to solidify nanocapsules.

Double emulsion methods sequentially emulsify water and oil phases, enabling the encapsulation of hydrophilic or hydrophobic actives.

Emulsion coacervation utilizes coagulants or crosslinkers to form rigid shell structures and load proteins.

• Layer-By-Layer (LBL)

At CD Formulation, we sequentially adsorb oppositely charged polyelectrolytes onto a colloidal template core to build polymer layers in a highly controlled manner. Through LBL technology, we are able to encapsulate a variety of active substances at designated locations within the nanocapsule layer. Furthermore, the release kinetics can be precisely tuned by adjusting the shell thickness, composition, and assembly sequence of the polymer.

How Do We Characterize Nanocapsules?

• We use dynamic light scattering (DLS) and zeta potential to determine size distribution and surface charge.
• Microscopy methods like scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) visualize morphology and internal structure.
• Stability is evaluated by monitoring drug content, zeta potential and size changes over time and under different conditions using DLS, zeta potential, chromatography or spectrometry.
• Encapsulation efficiency is determined by quantifying loaded drugs after isolation via centrifugation or ultrafiltration using HPLC, fluorescence or UV-Vis spectroscopy.
• In vitro release kinetics are measured by ultracentrifugation, centrifugal ultrafiltration or dialysis and fitted to mathematical models.

Advantages of Our Nanocapsule Development Services for Protein and Peptide Delivery

• The nanocapsules can be designed to offer controlled release of proteins and peptides, ensuring sustained and targeted delivery over an extended period.
• The encapsulation of proteins and peptides within nanocapsules enhances their bioavailability by protecting them from enzymatic degradation and improving their absorption and uptake in the body.
• We can customize the nanocapsules' size, surface properties, and release kinetics to meet the specific requirements of protein and peptide delivery, offering tailored solutions for different therapeutic applications.

At CD Formulation, we offer cutting-edge nanocapsule development services specifically designed for the efficient delivery of peptides and proteins. Our team of experts combines expertise in materials science, biology, and medicine to develop biocompatible nanocapsules. Through a comprehensive drug delivery system evaluation process, we ensure the effectiveness and safety of our nanocapsules. Contact us and let's set up an exploratory consultation!