Immunoprecipitation (IP)&Co-immunoprecipitation (Co-IP) Detection Service

Immunoprecipitation (IP) is a method based on the principle of antibody-antigen interaction, which is used to specifically enrich and separate proteins of interest and their interacting molecules. Its basic principle is to combine the target protein with a specific antibody to form an immune complex, and then use the affinity of the antibody to the immune complex to precipitate it together with other non-specific proteins. Through steps such as washing and centrifugation, non-specific proteins can be removed, thereby purifying the protein of interest and its interacting molecules.

Co-immunoprecipitation (Co-IP) is a method used to study protein interactions based on the interaction between antibodies and antigens. The principle is to use the endogenous target protein of the cell as bait, and co-incubate the target protein antibody with the total cell protein to promote the formation of immune complexes. Then add protein A/G that can bind to the target protein antibody to form a "binding protein-target protein-target protein antibody" complex. After purification, the immune complex is separated by gel electrophoresis and combined with Western blot or mass spectrometry technology to achieve the purpose of purifying the target protein and qualitatively detecting the antigen or antibody. Compared with other research methods, co-immunoprecipitation detects the interaction between proteins under physiological conditions. Therefore, not only can the complex formed in the body be detected, but also false positives caused by overexpression of the target protein can be eliminated. Second, endogenous target proteins are processed, modified, and mature proteins, so modification-dependent protein interactions can also be detected. CD Formulation can provide immunoprecipitation (IP)/co-immunoprecipitation (Co-IP) detection services.

Fig.1 Schematic diagram of immunoprecipitation.

The Comparison of Immunoprecipitation (IP)/Co-immunoprecipitation (Co-IP)

Our Service

• Experiment design based on your specific project needs;
• Parameters optimization, such as incubating time, elution strength, etc.;
• Cell lysis, Co-IP, washing & elution;
• Western blot and/or LC-MS/MS, depending on the project needs;
• Data analysis and report delivery

CD Formulation's Service Advantages

• Provide the highest quality lysis solution to retain the natural protein structure to the greatest extent.
• Provide optimized fillers to ensure maximum protein binding.
• A technical team that has been engaged in IP-related testing for a long time and has rich practical experience.
• Mature experimental mechanism and scientific control group setting maximize the accuracy of experimental results.

CD Formulation's immunoassay team has extensive experience in immunoprecipitation (IP)/co-immunoprecipitation (Co-IP). Our team of highly skilled scientists and engineers is committed to providing you with the highest quality service to meet your specific research and needs. Thank you for choosing our company's immunoprecipitation (IP)/co-immunoprecipitation (Co-IP) testing services. If you have any related needs, please contact our staff and we will answer and serve you immediately.