Immunofluorescence (IF) Detection Service

Immunofluorescence technology is a technology based on immunology, biochemistry and fluorescence imaging systems. Immunofluorescence technology is based on the specific combination of antibodies and anti-antibodies. Fluorescent pigments that do not affect the activity of antigens and antibodies are labeled on the antibodies. After combining with their corresponding antigens, a specific fluorescence reaction is displayed under a fluorescence microscope, which can be seen using a fluorescence microscope. The cells or tissues where the fluorescence is located determine the nature and location of the antigen. Immunofluorescence technology is widely used in biology and medicine such as immunology, microbiology, pathology, oncology and clinical testing due to its advantages of strong specificity, high sensitivity and easy operation. CD Formulation can provide high-quality immunofluorescence detection services.

The Features of Immunofluorescence (IF)

The main features of this technology are strong specificity, high sensitivity and fast speed. Compared with radioimmunoassay, immunofluorescence(IF) has no radioactive contamination, and most of them are simple to operate and easy to promote.

Our Immunofluorescence (IF) Detection Methods

CD Formulation has mastered many types of immunofluorescence (IF) methods, including:

• Direct Immunofluorescence (IF) - Labeled specific fluorescent antibodies are directly added to the antigen specimen, stained at a certain temperature and time, and excess fluorescent antibodies that have not participated in the reaction are washed away with water, dried at room temperature, and then sealed and examined under a microscope.
• Indirect Immunofluorescence (IF) - If unknown antigen is examined, the known unlabeled specific antibody (the first antibody) is first reacted with the antigen specimen, the unreacted antibody is washed with water, and then the labeled anti-antibody (the second antibody) is reacted with the antigen specimen to form an antibody-antigen-antibody complex. And the unreacted labeled antibody is washed with water, dried and sealed, and then examined under microscope. If an unknown antibody is tested, it indicates that the antigen specimen is known, the serum to be tested is the first antibody, and the other steps of the antigen test are the same.
  The labeled anti-antibody is an anti-globulin antibody, which has the same species specificity as serum globulin. For example, the immune anti-chicken serum globulin only reacts to chicken globulin. Therefore, the preparation of labeled antibodies is suitable for the diagnosis of any antigen.

Fig.1 Schematic diagram of Immunofluorescence (IF)

CD Formulation's Service Advantages

CD Formulation has professional technicians and confocal microscopes and other instruments to provide customers with high-quality immunofluorescence detection services. The experimental personnel have rich operating experience, and the test results are accurate and reproducible.

CD Formulation's immunoassay team has extensive experience in immunofluorescence (IF). Our team of highly skilled scientists and engineers is committed to providing you with the highest quality service to meet your specific research and needs. Thank you for choosing our company's immunofluorescence (IF) testing services. If you have any related needs, please contact our staff and we will answer and serve you immediately.