Enzyme-linked immunosorbent assay (ELISA), also known as enzyme immunoassay, or enzyme-linked assay for short, uses the specific binding of antibodies and antigens to conduct qualitative and quantitative detection of immune reactions. Monoclonal and polyclonal antibodies can be used to detect analytes such as peptides, proteins, antibodies, and small molecules. Antibodies develop specificity for the analyte, and a portion directly or indirectly couple to the antibody, thus providing a detection method and possible signal amplification. A variety of ELISA methods and assay chemistries allows the end user to tailor the appropriate assay to address anticipated experimental issues. ELISA is a classic experiment in immunology. Since its advent in the 1970s, it has developed rapidly due to its advantages such as high sensitivity, strong specificity, and good repeatability. It is currently widely used in many fields such as immunodiagnosis, detection, and biology. CD Formulation can provide ELISA detection services.
The Principle of ELISA
This is usually done with a 96-well plate capable of passively binding proteins. The corresponding antibody is prepared by coupling an enzyme. Adding this conjugate to the sample allows the antibody to bind to the antigen. Bound substances that are not specific for the antigen of interest can be subsequently removed using washing steps. The enzyme's substrate is then added, allowing the substrate in solution to react with the antibody-linked enzyme, resulting in a color, fluorescence, or luminescence change that can be read to determine the amount of target in each sample.
Our ELISA Detection Methods
CD Formulation has mastered many types of ELISA methods, including:
- Direct ELISA - The antigen is immobilized on an ELISA plate, and then an enzyme-labeled antibody is used to directly detect the antigen. Direct ELISA has fewer experimental steps, faster detection, does not require the use of secondary antibodies, avoids cross-reaction, and the detection results are not prone to errors.
- Indirect ELISA - Similar to direct ELISA, but without conjugated antibodies. Bound antibodies are detected using a secondary conjugated antibody. Compared with direct ELISA, indirect ELISA uses enzyme-labeled secondary antibodies, which has higher sensitivity, requires less labeled antibodies, and is more economical. Indirect ELISA also provides greater flexibility.
- Sandwich ELISA- The antigen is recognized by 2 antibodies, forming a sandwich-like complex. One antibody is used to capture and one is used to detect. Detection can be direct or indirect.
- Competitive/Inhibitory ELISA - Similar to direct ELISA, but quantification is performed by having the antibody competitively or inhibiting the antibody binding to a measured amount of antigen.
CD Formulation's Service Advantages
CD Formulation has a huge product library: a variety of high-quality ELISA application antibodies and ELISA kits, experienced experimental personnel, and experimental results are guaranteed; it is equipped with advanced instruments and equipment to ensure the smooth progress of the entire experiment.
CD Formulation's immunoassay team has extensive experience in ELISA detection service. Our team of highly skilled scientists and engineers is committed to providing you with the highest quality service to meet your specific research and needs. Thank you for choosing our company's ELISA detection service. If you have any related needs, please contact our staff and we will answer and serve you immediately.
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